Latex Aglutination (LAT) Test for detection of Toxoplasma gondii antibodies in sera of naturally infected pigs in District of Saranda, Albania

Artan Xhafa, Keti Margariti, Sonila Çoçoli, Natalia Shoshi, Ilirian Kumbe

Abstract


The aim of study was to determine the of Toxoplasma gondii antibodies in sera of naturally infected pigs in District of Saranda, Albania. Was collected 184 samples of pigs sera from  extensive farms at the time of slaughter in slaughterhouses. Collected blood was left to express serum and it was kept in a freezer until the time when samples were processed in the laboratory of Microbiology  of  Faculty of Veterinary Medicine of Tirana, Albania. Serum samples were examined by Latex aglutination test (LAT). Out of  184 samples tested, 25 sera had anti-Toxoplasma IgG antibodies. Average prevalence was about 13.97%.

Keywords:  Toxoplasma gondii, pigs, Latex aglutination test, pig sera.


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References


Cambiaso CL, Galanti LM, Leautaud P, Masson PL. Latex agglutination assay of human immunoglobulin M antitoxoplasma antibodies which uses enzymatically treated antigen-coated particles. J Clin Microbiol. 1992;30:882–8.

Dubey, J.P., Jones, J.L., 2008. Toxoplasma gondii infection in humans and animals in the United States. Int. J. Parasitol. 38, 1257–1278.

Dubey, J.P. 2009. Toxoplasmosis in pigs—The last 20 years. Veterinary Parasitology 164 (2009) 89–103

Edelhofer, R., 1994. Prevalence of antibodies against Toxoplasma gondii in pigs in Austria- an evaluation of data from 1982 and 1992. Parasitol.] Res. 80, 642–644.

Hassan Hajian-Bidar, Heidar Heidari, Aliasghar Bahari, Jamal Gharekhani. Sero-prevalence of Toxoplasma gondii infection in domestic goats in western Iran. International Journal of Advanced Research Volume 2, Issue 8, 639-643, 2014

Gamble, H.R., Brady, R.C., Dubey, J.P., 1999. Prevalence of Toxoplasma gondii infection in domestic pigs in the New England states. Vet. Parasitol. 82, 129–136.

Holliman RE, Barker KF, Johnson JD. Selective antenatal screening for toxoplasmosis and the latex agglutination test. Epidemiol Infect. 1990;105:409–14.

Kijlstra, A., Eissen, O.A., Cornelissen, J., Munniksma, K., Eijck, I., Kortbeek, T., 2004. Toxoplasma gondii infection in animal-friendly pig production systems. Invest. Ophthalmol. Vis. Sci. 45, 3165–3169.

Kijlstra, A., Meerburg, B., Cornelissen, J., De Craeye, S., Vereijken, P., Jongert, E., 2008. The role of rodents and shrews in the transmission of Toxoplasma gondii to pigs. Vet. Parasitol. 156, 183–190.

Liu Q, Singla LD, Zhou H. Vaccines against Toxoplasma gondii: status, challenges and future directions. Hum Vacc Immunother. 2012;8:1305–8.

Mazumder P, Chuang HY, Wentz MW, Wiedbrauk DL. Latex agglutination test for detection of antibodies to Toxoplasma gondii. J Clin Microbiol. 1988;26:2444–6.

Meerburg, B.G., van Riel, J.W., Cornelissen, J.B., Kijlstra, A., Mul, M.F., 2006. Cats and goat whey associated with Toxoplasma gondii infection in pigs. Vector-Borne Zoonotic Dis. 6, 266–274

Sato K, Ise Y, Iida T, Suzuki T, Shimada K, Nishioka K. Detection of toxoplasma IgM antibody by passive latex agglutination reaction. J Immunol Methods. 1987;101:183–91.

van der Giessen, J., Fonville, M., Bouwknegt, M., Langelaar, M., Vollema, A., 2007. Seroprevalence of Trichinella spiralis and Toxoplasma gondii in pigs from different housing systems in The Netherlands. Vet. Parasitol. 148, 371–374.

Zhou D H, R Liang C.C. Yin, F.R Zhao Z G. Yuan R Q. Lin H Q. Song and X Q. Zhu, 2010. Seroprevalence of Toxoplasma gondii in Pigs from Southern China J. Parasitol., 96: 673-674.




DOI: http://dx.doi.org/10.0001/ijllis.v7i2.1643

DOI (PDF): http://dx.doi.org/10.0001/ijllis.v7i2.1643.g2114

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