Genetic Characterization of Resistant S. Aureus Strains Isolated From Cow’s Milk with Mastitis

Keti Margariti, Pranvera Çabeli, Domenico Buonavoglia, Jonida Boci, Sonila Cocoli, Tana Shtylla, Aida Dama

Abstract


Mastitis therapy is generally unsuccessful due to pathological changes that occur in the breast as a result of the inflammatory response, factors associated with the causes of mastitis, the pharmacokinetic properties of antimicrobial drugs, etc. The main reason for the low effectiveness of antibiotic treatment of mastitis caused by staphylococci is especially, among other things, the resistance of bacteria, where is recognized that Staphylococcus aureus is the first penicillin-resistant bacteria. Laboratory techniques based on phenotypic characteristics of resistance have low sensitivity. PCR (Polymerase Chain Reaction) as a method for detecting gene mecA is considered as gold standard test. Detection of mecA gene through PCR is one of the most effective methods for the detection of MRSA. Using this method we identified six methicillin-resistant strains of S. aureus isolated from cow’s milk with mastitis. Our study presents the first survey carried out in our country regarding the presence of the mecA gene for antibiotic-resistant strains of S. aureus which is isolated from cow’s mastitis. This study has a special importance for food products of animal origin, like milk contaminated with MRSA may constitute a main source of consumer’s infection.

Keywords: MRSA, cattle, antibiogram, mecA.


Full Text:

PDF


DOI: http://dx.doi.org/10.0001/(aj).v3i8.303

DOI (PDF): http://dx.doi.org/10.0001/(aj).v3i8.303.g1515

Copyright © 2012-2019 ANGLISTICUM. Journal of the Association-Institute for English Language and American Studies,Tetovo, Republic of North Macedonia.

All Rights Reserved.

The publication is licensed under a Creative Commons License (CC BY) https://creativecommons.org/licenses/by/4.0/  View Legal Code                                                                                                                 

ISSN (print): 1857-8179. ISSN (online): 1857-8187.

Disclaimer: Articles on Anglisticum have been reviewed and authenticated by the Authors before sending for the publication.

The Journal, Editors and the editorial board are not entitled or liable to either justify or responsible for inaccurate and misleading data if any. It is the sole responsibility of the Author concerned.