Characterization and Toxicity of Bacillus Thuringiensis Strain Kfr-24, And Commercial Formulations against Spodoptera Littolaris

Hassan A. Ahmed, Salah G. Ali, Usama M. Abdul-Raouf

Abstract


Under these studies Bacillus thuringiensis wettable powder comparable to standard strain, there are only one isolate which confirmed by molecular characterization. Scanning electron microscopy observed that the presence of cuboidal crystals in Bacillus thuringiensis Alex-13, and observed bipyramidal crystals in case of Bacillus thuringiensis HD-1, as standard strain. Polymerase chain reactions (PCR) revealed the presence of Cry1-like sequences in addition to standard strain, which confirmed by gel electrophoresis. Also, during these studies.Bioassay recorded that the highest mortality rate
by determination of LC50 for 5 days against Spodoptera littolaris third instars larvae were 69, 86, and 100(%) in case of Bt Alex-13, Bt-HD-1 standard strain and Bt commercial wettable powder, respectively.

Full Text:

PDF


DOI: http://dx.doi.org/10.0001/(aj).v3i0.723

DOI (PDF): http://dx.doi.org/10.0001/(aj).v3i0.723.g889

Copyright © 2012-2021 ANGLISTICUM. Journal of the Association-Institute for English Language and American Studies,Tetovo, Republic of North Macedonia.

All Rights Reserved.

The publication is licensed under a Creative Commons License (CC BY) https://creativecommons.org/licenses/by/4.0/  View Legal Code                                                                                                                 

ISSN (print): 1857-8179. ISSN (online): 1857-8187.

Disclaimer: Articles on Anglisticum have been reviewed and authenticated by the Authors before sending for the publication.

The Journal, Editors and the editorial board are not entitled or liable to either justify or responsible for inaccurate and misleading data if any. It is the sole responsibility of the Author concerned.