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Control for Oxytetracycline Residues in Cow’s Milk in Tetovo, Macedonia

Mensur Kamberi, Kapllan Sulaj


The analytical control for qualitative and quantitative oxytetracycline residues evaluation in cow’s milk was performed in 2012 in cattle farms in Tetovo, Macedonia. The fresh cow’s milk samples were collected from 43 milking cows' farms in Tetovo. This research was focused on detection of oxytetracycline residue in 123 fresh milk samples using ELISA test for qualitative evaluation. After this check, positive samples were frozen and analyzed with high-performance liquid chromatography (HPLC) method for qualitative evaluation of oxytetracycline residues. The control carried out by ELISA test confirmed that 3.2% (4/123) of raw milk samples were positive for oxytetracycline residues. The qualitative evaluation was carried out in all positive milk samples using HPLC. Positive samples had following values of quantity in 1-liter milk: 60ug/l, 120ug/l, 200ug/l, 1100ug/l (ppb). Regarding positive results confirmed treatment of milking cows with high doses of oxytetracycline in Tetovo remaining a serious risk for consumers.

Keywords: residues, oxytetracycline, milk, cows, Tetovo.

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                     © 2012-2023 ANGLISTICUM. Journal of the Association-Institute for English Language and American Studies,Tetovo, North Macedonia.

                                                                                           ISSN (print): 1857-8179. ISSN (online): 1857-8187.

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