CHECK OF WILD RABBIT MEAT IN MACEDONIA

Abstract

In this duty were performed the immunohistochemical examinations of 295 tissues and organs of suspected rabbits and 70 specimens from the tissues of the rabbits killed by the hunters in Macedonia in the regions of Dibresh, Nerove, Allbance, Presille, Bellushine, Haracine, Tearce etc.. These samples were collected from the shops and the restaurants which served rabbit meat in theor menu. The number of collected samples was considerable considering that the population of the above mentioned areas prefers mostly the consume of the wild rabbit meat. The meat of wild rabbit is consumed by the hunters and their families as well as some luxurious restaurants. The meat of wild rabbit in Macedonia is used also in touristic areas. The collected samples were sent at the SKE Lab at the Faculty of Veterinary Medicine in which were realized 70 formalin-fixed samples according to the protocol which accompanied the Kit. Based on the fat of the kidneys, the carcasses were analyzed in three categories (good, moderated weak) The tissue samples were collected to be examined fresh or stored in 10% buffered formalin for histological and immunohistochemical examination. From the organ samles were prepared microscopic stripes that were stained with the classic hematoxylin eosin and ABC method. The prepared stripes were examined in a light microscope laboratory. Immunohistochemistry (IHC) model Avidin Biotin Complex (ABC), IHC-ABC, was applied and detected by microscopy the presence of F-tularensis lipopolysaccharide antigens in the rabbit tissue sections. Positive with a lesion in the organ resulted 12 samples (0.6%) of the examined tissues with immunohistochemistry were found to be be affected at least one of the observed organs. The total of the immunohistologic positive samples was dominated by the lung with 5 positive cases. The presence of macroscopy hotbeds was visible with white colour and in the nodules with diameter of 0,1-1,0 cm in special organs or more than one organ. The hunters should be warmed for the presence of tularemia. The presence of the macroscopically visible grey colored in white hotbeds in the nodules with diameters of  0.1 -1.0 cm in the body, more or less the same as the paralyzing agent and the agent for the presence of tularemia. The results of the immunohistochemical examination are presented considerably lower compared with the results of the same alternative, but in rabbit samples that resulted seropositive. Only 12 samples (0.6%) of the examined tissues with the immunohistocation of rabbits were found positive with a lesion in the organs where they were found to be affected at least in one of the observed organs.

Keywords: Wild rabbit, meat, samples, methods, Macedonia.