Latex Aglutination (LAT) Test for detection of Toxoplasma gondii antibodies in sera of naturally infected pigs in District of Saranda, Albania
Abstract
The aim of study was to determine the of Toxoplasma gondii antibodies in sera of naturally infected pigs in District of Saranda, Albania. Was collected 184 samples of pigs sera from extensive farms at the time of slaughter in slaughterhouses. Collected blood was left to express serum and it was kept in a freezer until the time when samples were processed in the laboratory of Microbiology of Faculty of Veterinary Medicine of Tirana, Albania. Serum samples were examined by Latex aglutination test (LAT). Out of 184 samples tested, 25 sera had anti-Toxoplasma IgG antibodies. Average prevalence was about 13.97%.
Keywords: Toxoplasma gondii, pigs, Latex aglutination test, pig sera.
References
Cambiaso CL, Galanti LM, Leautaud P, Masson PL. Latex agglutination assay of human immunoglobulin M antitoxoplasma antibodies which uses enzymatically treated antigen-coated particles. J Clin Microbiol. 1992;30:882–8.
Dubey, J.P., Jones, J.L., 2008. Toxoplasma gondii infection in humans and animals in the United States. Int. J. Parasitol. 38, 1257–1278.
Dubey, J.P. 2009. Toxoplasmosis in pigs—The last 20 years. Veterinary Parasitology 164 (2009) 89–103
Edelhofer, R., 1994. Prevalence of antibodies against Toxoplasma gondii in pigs in Austria- an evaluation of data from 1982 and 1992. Parasitol.] Res. 80, 642–644.
Hassan Hajian-Bidar, Heidar Heidari, Aliasghar Bahari, Jamal Gharekhani. Sero-prevalence of Toxoplasma gondii infection in domestic goats in western Iran. International Journal of Advanced Research Volume 2, Issue 8, 639-643, 2014
Gamble, H.R., Brady, R.C., Dubey, J.P., 1999. Prevalence of Toxoplasma gondii infection in domestic pigs in the New England states. Vet. Parasitol. 82, 129–136.
Holliman RE, Barker KF, Johnson JD. Selective antenatal screening for toxoplasmosis and the latex agglutination test. Epidemiol Infect. 1990;105:409–14.
Kijlstra, A., Eissen, O.A., Cornelissen, J., Munniksma, K., Eijck, I., Kortbeek, T., 2004. Toxoplasma gondii infection in animal-friendly pig production systems. Invest. Ophthalmol. Vis. Sci. 45, 3165–3169.
Kijlstra, A., Meerburg, B., Cornelissen, J., De Craeye, S., Vereijken, P., Jongert, E., 2008. The role of rodents and shrews in the transmission of Toxoplasma gondii to pigs. Vet. Parasitol. 156, 183–190.
Liu Q, Singla LD, Zhou H. Vaccines against Toxoplasma gondii: status, challenges and future directions. Hum Vacc Immunother. 2012;8:1305–8.
Mazumder P, Chuang HY, Wentz MW, Wiedbrauk DL. Latex agglutination test for detection of antibodies to Toxoplasma gondii. J Clin Microbiol. 1988;26:2444–6.
Meerburg, B.G., van Riel, J.W., Cornelissen, J.B., Kijlstra, A., Mul, M.F., 2006. Cats and goat whey associated with Toxoplasma gondii infection in pigs. Vector-Borne Zoonotic Dis. 6, 266–274
Sato K, Ise Y, Iida T, Suzuki T, Shimada K, Nishioka K. Detection of toxoplasma IgM antibody by passive latex agglutination reaction. J Immunol Methods. 1987;101:183–91.
van der Giessen, J., Fonville, M., Bouwknegt, M., Langelaar, M., Vollema, A., 2007. Seroprevalence of Trichinella spiralis and Toxoplasma gondii in pigs from different housing systems in The Netherlands. Vet. Parasitol. 148, 371–374.
Zhou D H, R Liang C.C. Yin, F.R Zhao Z G. Yuan R Q. Lin H Q. Song and X Q. Zhu, 2010. Seroprevalence of Toxoplasma gondii in Pigs from Southern China J. Parasitol., 96: 673-674.
Downloads
Published
How to Cite
Issue
Section
License
Declaration/Copyright transfer:
1. In consideration of the undertaking set out in paragraph 2, and upon acceptance by ANGLISTICUM for publication of the manuscript in the Journal, I/We hereby assign and transfer publication rights to ANGLISTICUM, whereas I/We retain the copyright for the manuscript. This assignment provides ANGLISTICUM the sole right and responsibility to publish the manuscript in its printed and online version, and/or in other media formats.
2. In consideration of this assignment, ANGLISTICUM hereby undertakes to prepare and publish the manuscript in the Journal, subject only to its right to refuse publication if there is a breach of the Author’s warranty in paragraph 4 or if there are other reasonable grounds.
3. Editors and the editorial board of ANGLISTICUM are empowered to make such editorial changes as may be necessary to make the Manuscript suitable for publication.
4. I/We hereby acknowledge that: (a) The manuscript submitted is an original work and that I/We participated in the work substantively and thus I/We hereby are prepared to take public responsibility for the work; (b) I/We hereby have seen and approved the manuscript as submitted and that the manuscript has not either been published, submitted or considered for publication elsewhere; (c) The text, illustration, and any other materials included in the manuscript do not infringe upon any existing copyright or other rights of anyone.
5. I/We hereby indemnify ANGLISTICUM and the respective Editors of the Journal as mentioned in paragraph 3, and hold them harmless from any loss, expense or damage occasioned by a claim or suit by a third party for copyright infringement, or any suit arising out of any breach of the foregoing warranties as a result of publication of the manuscript.
